En Es
Categories

Industry News


New Gold and Copper Nanoshell-Enhanced Immunoblotting Technique Makes POC Diagnosis of Tuberculosis Easier

By Labmedica International staff writers
09 Sep 2020

IllustrationA team of researchers have developed a straightforward and naked-eye-sensitive nanoshell-enhanced immunoblotting technique to detect Mycobacterium tuberculosis (MTB) antigen 85B (Ag85B).

The group of researchers from DxGen Corporation (Gunpo-si, Gyeonggi-do, Korea), Chung-Ang University, ASAN Medical Center, and Choongnam National University Medical School has developed a novel gold binding peptide (GBP) that specifically binds to Ag85B secreted from MTB. Also, a new Cu or Gold ion treatment method was proposed to intensify the colorimetric signal of gold nanoparticles (AuNP). Research has revealed that the economical paper-based colorimetric detection method can easily diagnose active TB with only a small amount of urine sample with high sensitivity.

The process involves sequentially dropping a urine sample containing the secretion of MTB and the AuNP-GBP conjugate onto a piece of nitrocellulose membrane paper. After that, a solution containing Cu or Gold ions is dropped on the membrane that combines with AuNPs to form a new ‘shell-shape’ nanostructure by changing the size and shape. This results in enhancing the colorimetric signal to make it strong enough to easily be detected by the naked eye. Since the developed immunoassay directly detects the antigen, it can significantly reduce false positives caused by latent TB or a past infection. Furthermore, Cu- or Gold ion treatment intensifies the colorimetric signal by 20 times, making it stronger to detect very low concentrations even with a smartphone or optical analyzer (0.93 ng/ml and 0.21 ng/ml for Cu- or Gold ion treatment, respectively).

A clinical study conducted for 52 urine samples composed of active TB, latent TB, NTM and non-infected subjects showed that the infected samples had either Ag85B or CFP10 antigens. The results demonstrated that the developed assay has a sensitivity and specificity of 71.4% and 86.7%, respectively.

"The biggest challenge in detecting POCT TB is to generate signals strong enough to be detected by inexpensive systems. By using the technology we developed, qualitative detection is possible with the naked eye, so that it can be used in low-resource countries," said Dr. Prof. Tae-Jung Park from the Chungang University, the corresponding author of the published paper.

"To implement a cost-effective product to help ending TB in various countries, we will commercialize the research results by implementing on the Epithod(R) analyzer and test kit platform," said Dr. Jinwoo Lee, CEO of DxGen Corp.




E-mail Print
FaceBook Twitter Google+ Linked in

DxGen Corp.

DxGenco is Point-Of-Care (POC) IVD company focused on developing multi-markers for people with diabetes and tuberculosis. It provides total solutions for POC diagnostics and offers full service for developing POC products from concept through commercialization.
Gold member
More info

Additional news

16 Sep 2020
MEDICA and COMPAMED 2020 to Take Place Entirely Online From 16-19 November 2020
MEDICA 2020 and COMPAMED 2020, both leading information and communication platforms for the medical technology industry and supplier industry for the medical technology industry, will take place entirely online from 16 to 19 November.
Read More
03 Sep 2020
Arab Health Rescheduled to June 2021 Due to COVID-19 Pandemic
Arab Health, the largest gathering of healthcare and trade professionals in the MENA region, has been rescheduled from its traditional January timeline to 21-24 June 2021 due to the COVID-19 pandemic.
Read More
25 Aug 2020
2020 AACC Annual Scientific Meeting & Clinical Lab Expo to Be an All Virtual Event Due to Coronavirus Pandemic
The American Association for Clinical Chemistry (AACC Washington, DC, USA) has decided to hold the 2020 AACC Annual Scientific Meeting & Clinical Lab Expo as a virtual event, rather than as a live meeting, due to the ongoing coronavirus pandemic.
Read More
20 Aug 2020
Hologic Showcased Latest Advances at Virtual ECR Congress 2020
Hologic, Inc. (Marlborough, MA, USA) showcased its latest advances and shared its new innovations on its virtual booth at the online ECR 2020 event held on July 15-21.
Read More
14 Aug 2020
QIAGEN to Focus on Molecular Testing amidst Coronavirus Pandemic after Voluntary Public Takeover Offer by Thermo Fisher Falls Short
QIAGEN N.V. (Venlo, Netherlands) plans to continue executing its growth strategy aimed at creating significant value for shareholders and other stakeholders after the voluntary public takeover offer by Thermo Fisher Scientific Inc. (Waltham, MA, USA) failed to achieve the minimum 66.67% acceptance threshold from QIAGEN shareholders.
Read More
03 Aug 2020
Siemens to Acquire Varian Medical to Create Comprehensive Cancer Portfolio
Siemens Healthineers (Erlangen, Germany) has entered into an agreement to acquire Varian Medical Systems, Inc. (Palo Alto, CA, USA) that will lead to the creation of a global leader in healthcare with a comprehensive portfolio to fight cancer.
Read More
17 Jul 2020
Awareness Technology Announces ChemWell RPR Analyzer - The Most Significant Innovation in Syphilis Testing in Last 30 Years
Awareness Technology, Inc. (Palm City, FL, USA) has announced the most significant innovation in syphilis testing in the last 30 years with the launch of the ChemWell RPR automated nontreponemal analyzer for the detection of syphilis.
Read More
23 Jun 2020
Omnia Health Live Unites Global Healthcare Community Through New Virtual Experience
Omnia Health Live, a virtual healthcare event, has brought the global healthcare community together on a scale never seen before through a new experience delivered entirely online.
Read More
12 Jun 2020
Global Magnetic Resonance Imaging (MRI) Market to be driven by Advancements in Medical Diagnostics
The global magnetic resonance imaging (MRI) market was valued at USD 5.90 billion in 2017 and is projected to register a CAGR of 5.2% from 2018 to 2026, driven by increased demand for MRI equipment across the world due to a substantial rise in incidence of disorders related to spine, neurology, orthopedic, cardiovascular, head and neck, and abdominal and prostate.
Read More
Copyright © 2000-2020 TradeMed.com. All rights reserved. | Terms And Conditions