Description
The FV3000 Confocal Laser Scanning Microscope features the high sensitivity and speed required for live cell imaging as well as deep tissue observation, enabling a wide range of imaging modalities, including macro-to-micro imaging, super resolution microscopy, and quantitative data analysis. It allows choosing between upright and inverted microscope frames suited for a range of life science applications, including developmental biology, stem cell research, electrophysiology, cancer research, slide imaging, and more.
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Features
GUI designed for darkroom environment. User-arrangeable layout.
Acquisition parameter reload features. Hard disk recording capability; adjust laser power and HV with Z-stack acquisition.
Z-stack with alpha blending, maximum-intensity projection, iso-surface rendering.
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Specifications
• Main Laser Combiner Violet/Visible Light Laser 405 nm: 50 mW, 488 nm: 20 mW, 561 nm: 20 mW, 640 nm: 40 mW
One optional laser port for the sub laser combiner or optional laser unit
• Optional Laser Sub Laser Combiner Maximum 3 laser units as follows:
445 nm: 75 mW, 514 nm: 40 mW, 594 nm: 20 mW, fiber connected to main laser combiner
Single Laser Unit 445 nm: 75 mW, 514 nm: 40 mW, or 594 nm: 20 mW, directly connected to main laser combiner
• Laser Light Control Main laser combiner with implemented AOTF system, ultra-fast intensity modulation with individual laser lines, additional shutter control continuously variable (0.1%–100%, 0.1% increments)
10% or 100% maximum laser power using an ND filter
• Scanner Scanning Method 2 silver-coated galvanometer scanning mirrors
Galvanometer Scanner (Normal Imaging) Scanning Resolution: 64 × 64 to 4096 × 4096 pixels
Scanning Speed (One Way): 512 × 512 with 1.1 s–264 s. pixel time: 2 µs–1000 µs
Scanning Speed (Round Trip): 512 × 512 with 63 ms–250 ms, 256 × 256 with 16 ms–125 ms
Optical Zoom: 1X–50X in 0.01X increments
Scan Rotation: Free rotation (360 degree) in steps of 0.1 degree
Scanning Mode: PT, XT, XZ, XY, XZT, XYT, XYZ, XYλ, XYZT, XYλT, XYλZ, XYλZT
ROI scanning, rectangle clip, ellipse, polygon, free area, line, free line and point, tornado mode only for stimulation
Resonant Scanner (High-Speed Imaging) -
Pinhole Single motorized pinhole, pinhole diameter ø50–800 µm (1 µm steps)
Field Number (FN) 18
Dichromatic Mirror Turret 8 positions (high-performance DMs and 10/90 mirror)
Optional Unit for Scanner Laser power monitor, optional laser port
• High-Sensitivity Spectral Detector Detector Module Cooled GaAsP photomultiplier, 2 channels
Spectral Method Motorized volume phase holographic transmission diffraction grating, motorized adjustable slit,
selectable wavelength bandwidth: 1–100 nm, wavelength resolution: 2 nm
Dichromatic Mirror Turret 8 positions (high-performance DMs and mirror)
• Spectral Detector Detector Module Multi-alkali photomultiplier, 2 channels
Spectral Method Motorized volume phase holographic transmission diffraction grating, motorized adjustable slit
selectable wavelength bandwidth: 1–100 nm, wavelength resolution: 2 nm
Dichromatic Mirror Turret 8 positions (high-performance DMs and mirror)
• System Control Control Unit OS: Windows® 7 Professional 64-bit (English version), Windows 10 Professional 64-bit ;
built-in dedicated I/F board and hardware sequencer for precise imaging timing
Display 30- or 32-inch monitor (WQUXGA 2560 × 1600)
• Fluorescence Illumination Unit External fluorescence light source, fiber adaptor to the optical port of the scan unit, motorized switching between the LSM light path and fluorescence illumination
• Transmitted Light Detector Unit Module with integrated external transmitted light photomultiplier detector and LED lamp, motorized switching
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