Description
Reliance One-Step Multiplex Supermix is designed for sensitive amplification of up to five targets in a single reaction. Expertly formulated with high-performance enzymes and advanced reaction buffer, this robust supermix delivers reproducible RT-qPCR performance in a multiplex fashion, including with challenging templates, difficult targets or in the presence of PCR inhibitors. With its exceptional room temperature stability for assembled reactions, it can be easily integrated into an automated workflow. Reliance One-Step Multiplex Supermix is an ideal solution for multiplexed RT-qPCR, especially for pathogen/viral detection and high throughput screening applications.
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Features
• Sensitive, reproducible multiplexing of up to 5 targets — get the most out of your samples while reducing costs
• 24-hour assembled reaction stability at room temperature — enables the use in an automated workflow to maximize throughput
• Convenient one-tube supermix — for minimizing reaction setup time and handling errors
• 4x concentrated formulation — increase detection sensitivity by maximizing input volume
• Tolerant to common PCR inhibitors — for use with lysates or samples of varying quality
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Specifications
Product format Single-tube, ready-to use supermix
Concentration 4x concentrated
Detection chemistry Probes
Multiplex capability Up to 5 targets
Assembled reaction stability Up to 24 hours
Inhibitor tolerance Tested with ethanol and hematin. Also compatible with up to 10 µl of crude lysates
5’ to 3’ exonuclease activity of the enzyme(s) Yes
Hot-start, activation time Yes, 10 min
Sample input 1 pg to 1 µg
Sensitivity 10 copies in a 5-plex reaction
Reference dye Tented universal dye preblended in the supermix
Instrument compatibility Both ROX-dependent (high- and low-) and -independent instruments
Guaranteed shelf life at –20° 12 months
Shelf life at 4°C (after thawing) 1 week
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