The Nano-Check 4 IN 1 Test is a rapid immunoassay for the determination of Cardiac Troponin I (cTnI), Creatine Kinase MB (CK-MB), Myoglobin, and NT-proBNP in human whole blood, serum, and plasma specimens at a reference level of 0.5 ng/ml, 5.0 ng/ml, 80 ng/ml, and 125 pg/ml for patients younger than 75 years or 450 pg/ml for patients 75 years and older, as an aid in the diagnosis of cardiac disease. The test monitors the rise and fall of cTnI, CK-MB, Myoglobin, and NT-proBNP in conjunction with the Nano-Checker 710 reader.